The number of CRP repeats is the major feature differentiating the type of selectin in extracellular region. In human, P-selectin has nine repeats while E-selectin contains six and L-selectin has only two. P-selectin is anchored in transmembrane region that is followed by a short cytoplasmic tail region.

1990-08-01 · View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR016187, CTDL_fold IPR000742, EGF-like_dom IPR033991, Selectin_CTLD IPR002396, Selectin_superfamily IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom: Pfam i

PECAM-1 p < 0.05 vs. Negative control. E-selectin, along with P-selectin and L-selectin, initiate recruitment of circulating leukocytes from blood to sites of inflammation in the vascular lining through Endothelial staining for E-selectin and the finding of iNOS and eNOS in SSc skin 1, E-selectin and P-selectin seems to play an important role in the homing of Cyclic GMP is produced in the VSMC by guanylate cyclase upon its activation Förändringarna i perifera blodnivåer av TNF-α, IL-6, MCP-1, sICAM-1, sVCAM-1, vWF, E-selectin, P-selectin, Endothelin-1, PAI-1, tPA och HMW Adiponectin vid av C Lonati · 2020 · Citerat av 1 — E-selectin and VCAM-1-positive vessels Melanocortins could reinforce endogenous signals providing protection versus respiratory Varga Z, Flammer AJ, Steiger P, Haberecker M, Andermatt R, Zinkernagel AS, et al. We suspected that E-selectin ligands might contribute to the remaining in that E-selectin ligands cooperate with alpha4 integrin rather than P-selectin ligands. Leukocyte rolling on the vessel wall is mediated by members of the selectin and P selectin (CD62P) and inducible E-selectin (CD62E) are expressed on endothelial cells. 1,2 P-selectin is stored in an inactive form in a-granules of platelets and in the Weibel-Palade body of endothelial cells. pb/0.05 vs Control group.

Blocking of selectins is therefore considered a promising E- and P-selectin: differences, similarities and implications for the design of P-selectin antagonists. Selectins form a family of Ca2+ -dependent carbohydrate binding proteins that mediate the initial step of leukocyte recruitment in the inflammatory process. Blocking of selectins is therefore considered a promising therapeutic approach to treat P-selectin, the largest selectin, is stored in α-granules of platelets and in Weibel–Palade bodies of endothelial cells, and is translocated to the cell surface of activated endothelial cells and platelets. E-selectin is not expressed under baseline conditions, except in skin microvessels, but is rapidly induced by inflammatory cytokines. During P- and E-selectin-mediated migration of Tc1 cells, P-selectin glycoprotein ligand-1 appears to be the sole ligand for P-selectin and one of the ligands for E-selectin. P- and E-selectin-independent migration of Tc1 cells into the inflamed skin was predominantly mediated by L-selectin.

When endothelial cells are activated by molecules such as histamine or thrombin during inflammation, P-selectin moves from an internal cell location to the endothelial cell surface. Se han identificado a tres miembros de esta familia, los cuales corresponden a los antígenos de diferenciación leucocitaria CD62L (L-selectina), CD62P (P-selectina) y CD62E (E-selectina); estas tres moléculas reconocen y se unen, a través de su dominio tipo lectina, a diversos oligosacáridos, los cuales están usualmente conjugados con proteínas transmembranales.

This together with minor differences in fucose binding, accounts for the 10-fold higher affinity of E-selectin for SLe x as compared to P-selectin. However, although P-selectin binds SLe x only with millimolar affinity (K D = 7.8 mM), very high affinities are obtained for specific glycoprotein counterreceptors (800 nM for SLe x-modified PSGL-1).

Puri- fied P-selectin inhibited CH0:P-selectin binding to HL6O cells, but incompletely inhibited CH0:E-selectin binding to HL60 cells. However, purified soluble E- selectin inhibited CH0:P-selectin and CH0:E-selectin binding to HL60 cells equivalently and completely. This together with minor differences in fucose binding, accounts for the 10-fold higher affinity of E-selectin for SLe x as compared to P-selectin.

av L Dieterich · 2011 · Citerat av 2 — teractions by increasing surface E-selectin. Manuscript. IV Dieterich L.C. teins facilitating leukocyte-endothelial adhesion, including P-selectin and von. Willebrand factor, but rolling speed in vivo (24 h versus 4 h stimulation). Future plans.

1,2 P-selectin is stored in an inactive form in a-granules of platelets and in the Weibel-Palade body of endothelial cells. pb/0.05 vs Control group.

12,13 E-selectin is not expressed constitutively in normal tissues but is transcriptionally up-regulated by proinflammatory to a lesser extent, E-selectin-mediated binding. Puri- fied P-selectin inhibited CH0:P-selectin binding to HL6O cells, but incompletely inhibited CH0:E-selectin binding to HL60 cells. However, purified soluble E- selectin inhibited CH0:P-selectin and CH0:E-selectin binding to HL60 cells equivalently and completely.
Mora - vita hasten

Short name: GMP-140 1 Publication The selectin family members, L-Selectin, P-Selectin, and E-Selectin, are involved in the adhesion of leukocytes to activated endothelium. This adhesion is 2b) (P < 0.05 versus LPS-Gal plus PBS, n = 7 to 9). Again, neither the anti-E- nor the anti-L-selectin antibody altered leukocyte adhesion.

Function . E-selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a selectin cell adhesion molecule expressed only on endothelial cells activated by cytokines.Like other selectins, it plays an important part in inflammation.In humans, E-selectin is encoded by the SELE gene. L-1 also plays a crucial role in T-cell homeostasis by binding to lymphoid chemokines, and can induce tolerance by enhancing the functions of regulatory T cells.
Bilaga

P-Selectin cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each P-Selectin of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.

P-selectin was first identified in 1984, using antibodies raised against activated platelets [1, 2]. In these initial publications, P-selectin was described as a protein of molecular weight of P-selectin is constitutively expressed and stored in Weibel-Palade bodies. 8 When stimulated, P-selectin is translocated and up-regulated immediately, initiating the earliest phase of leukocyte recruitment into inflammatory sites. 8 Moreover, this molecule can be regulated transcriptionally by proinflammatory cytokines, such as tumor necrosis factor (TNF)–α. 12,13 E-selectin is not expressed constitutively in normal tissues but is transcriptionally up-regulated by proinflammatory to a lesser extent, E-selectin-mediated binding. Puri- fied P-selectin inhibited CH0:P-selectin binding to HL6O cells, but incompletely inhibited CH0:E-selectin binding to HL60 cells.